The chapters are in-depth discussion by experts on both bioinformaticians general techniques and specific approaches to various problems of bioinformatics is selected. data mining, machine learning, mathematical modeling, sequence alignment, data integration, workflow development, etc. In-depth discussion of computing recognition and regulation of functional sites in DNA sequences. Sharp discussion of the computational prediction of RNA secondary structure. Overview of the computational prediction of protein cellular localization, and discussion of selected conclusions protein function. Detailed discussion of approaches to the analysis of gene expression for disease diagnosis, treatment, and understanding of gene function. Case studies on the phylogeny analysis, protein functional explanation, the construction of purpose-built integrated biological databases, and workflows that underlie the development of large-scale gene discovery efforts.