Sequel to the popular lecture titled book Biomedical Image Analysis: Tracking, this book on Biomedical Image Analysis: Segmentation handle the challenging task of biological and medical image segmentation. Model active methods that emerged in recent years is the focus of this book, including parametric active contour and active surface models, active shape model, and geometric active contours adapt to the topology of the image. Contents: Introduction / Parametric Active Contour / Active Contours in Bayesian Framework / Geometric Active Contours / Segmentation by Graph Algorithms Image / Scale-Space Filtering for the Segmentation